Study of protein corona for polymeric drug delivery systems

Speaker: Sergey Filippov, Harvard University

When: March 22, 2019 (Fri), 12:00PM to 01:00PM (add to my calendar)
Location: SCI 352
Hosted by: Rama Bansil

This event is part of the Biophysics/Condensed Matter Seminar Series.

Polymer drug carriers based on N-(2-Hydroxypropyl)methacrylamide (HPMA) copolymers bearing cholesterol moieties have been studied extensively over the past few years, to understand the aggregation behavior and shape of the copolymers in dilute aqueous solutions [1,2]. Above certain concentration, micelle-like nanoparticles (NPs) having ellipsoidal shape are observed. However, there is a lack of knowledge on the behavior of these drug carriers in human blood environment. Human serum albumin (HSA), fibrinogen, Imunoglobulin G, and apolipoproteins A-E were chosen for this study as basic proteins, which are the main component of human blood plasma. We use fluorescence life-time correlation spectroscopy (FLCS), Electron Paramagnetic Resonance (EPR), isothermal titration calorimetry (ITC), nuclear magnetic resonance (NMR), and small angle X-ray scattering (SAXS) to investigate HPMA copolymers that are dissolved in an aqueous solution of proteins to reveal the interaction between the NPs and proteins [3,4].

[1] S. K. Filippov, et al., Biomacromolecules, 2012, 13, 2594 [2] S. K.Filippov, et al., Biomacromolecules, 2013, 14, 4061 [3] Xiaohan Zhang, ,,, Sergey K. Filippov, Christine M. Papadakis, Langmuir, 2018, 34 (27), 7998-8006. [4] Damir Klepac,,,,, Sergey K. Filippov, Nanoscale, 2018, 10(13), 6194-6204.