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Selected Journal Publications:

M Lim, KJ Rothschild:
Photocleavage-Based Affinity Purification and Printing of Cell-Free Expressed Proteins.
Analytical Biochemistry, 2008

JA Cappuccio, CD Blanchette, TA Suchek, ES Arroyo, JM Kralj, AK Hinz, EA Kuhn, BA Chromy, BW Segelke, KJ Rothschild, JE Fletcher, F Katzen, TC Peterson, WA Kudlicki, G Bench, PD Hoeprich, MA Coleman:
Cell-Free Co-Expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles.
Molecular & Cellular Proteomics 7:  2246-2253, 2008

VB Bergo, OA Sineschekov, JM Kralj, R Partha, EN Spudich, KJ Rothschild, JL Spudich:
His75 in Proteorhodopsin, a Novel Component in Light-Driven Proton Translocation by Primary Pumps.
J. Biol. Chem.:  E-publication 2008

JJ Amsden, JM Kralj, VB Bergo, EN Spudich, JL Spudich, KJ Rothschild:
Different Structural Changes Occur in Blue- and Green-Proteorhodopsins Suring the Primary Photoreaction.
Biochemistry 47 (44):  11490-11498, 2008

JM Kralj, EN Spudich, JL Spudich, KJ Rothschild:
Raman Spectroscopy Reveals Direct Chromophore Interactions in the Leu/Gln105 Spectral Tuning Switch of Proteorhodopsins.
J. Phys. Chem. B. 112 (37):  11770-11776, 2008

JM Kralj, VB Bergo, JJ Amsden, EN Spudich, JL Spudich, KJ Rothschild:
Protonation State of Glu142 Differs in the Green- and Blue-Absorbing Variants of Proteorhodopsin.  Biochemistry 47 (11):  3447-3453, 2008

JJ Amsden, JM Kralj, LR Chieffo, X Wang, S Erramilli, EN Spudich JL Spudich, LD Ziegler, KJ Rothschild:
Subpicosecond Protein Backbone Changes Detected during the Green-Absorbing Proteorhodopsin Primary Photoreaction.  J. Phys. Chem. B. 111 ( 40 ):  11824-11831, 2007

LR Chieffo J Shattuck, JJ Amsden, S Erramilli, LD Ziegler:
Ultrafast Vibrational Relaxation of Liquid H2O Following Vibrational Combination Band Excitation.  Chem. Phys. 341 (1-3): 71-80, 2007

VB Bergo, M Ntefidou, VD Trivedi, JJ Amsden, JM Kralj, KJ Rothschild, JL Spudich:
Conformational Changes in the Protocycle of Anabaena Sensory Rhodopsin:  Absence of the Schiff Base Counterion Protonation Signal.  J. Biol. Chem. 281 (22):  15208-15214, 2006

SK Maji, JJ Amsden, KJ Rothschild, MM Condron, DB Teplow:
Conformational Dynamics of Amyloid b-Protein Assembly Probed Using Intrinsic Fluorescence.  Biochemistry 44 (40):  13365-13376, 2005

J Olejnik, S Gite, S Mamaev, KJ Rothschild:
N-terminal labeling of proteins using initiator tRNA.  Methods 36:  252, 2005

VB Bergo, EN Spudich, KJ Rothschild, JL Spudich:
Photoactivation perturbs the membrane-embedded contacts between sensory rhodopsin II and its transducer.  J. Biol. Chem. 280 (31):  28365-28369, 2005

VB Bergo, JJ Amsden, EN Spudich, JL Spudich, KJ Rothschild:
Structural changes in the photoactive site of proteorhodopsin during the primary photoreaction.  Biochemistry 43 (28):  9075-9083, 2004

S Mamaev, J Olejnik, EK Olejnik, KJ Rothschild:
Cell-free N-terminal protein labeling using initiator suppressor tRNA.  Anal. Biochem. 326:  25, 2004

V Bergo, EN Spudich, JL Spudich, KJ Rothschild:
Conformational Changes Detected in a Sensory Rhodopsin II-Transducer Complex.  J. Biol. Chem. 278 (38):  36556-36562, 2003

S Gite, M Lim, R Carlson, J Olejnik, B Zehnbauer, KJ Rothschild:
A high-throughput nonisotopic protein truncation test.  Nature Biotech. 21:  194-197, 2003

V Bergo, S Mamaev, J Olejnik, KJ Rothschild:
Methionine Changes in Bacteriorhodopsin Detected by FTIR and Cell-Free Selenomethionine Substitution.  Biophysical Journal 84:  960-966, 2003

S Gite, M Lim, R Carlson, J Olejnik, B Zehnbauer, KJ Rothschild:
A high-throughput nonisotopic protein truncation testNature Biotech. 21:  194-197, 2003

V Bergo, S Mamaev, J Olejnik, KJ Rothschild:
Methionine changes in bacteriorhodopsin detected by FTIR and selenomethionine incorporation.   Biophys. J. 84:  960-966, 2003

V Bergo, EN Spudich, JL Spudich, KJ Rothschild:
A Fourier Transform Infrared Study of Neurospora Rhodopsin:  Similarities with Archaeal Rhodopsins
Photochem. and Photobiol. 76 (3):  341-349, 2002

V Bergo, K Scott, E Spudich, JL Spudich, KJ Rothschild:
Light induces similar structural changes in archaeal sensory rhodopsin II and in a eukaryotic rhodopsin from Neurospora crassa.  Photochem. Photobiol. 76:  341, 2002

V Bergo, EN Spudich, KL Scott, JL Spudich, KJ Rothschild:
FTIR Analysis of the SRII540 Intermediate of Sensory Rhodopsin II: Asp73 is the Schiff Base Proton Acceptor
Biochemistry 39 (11):  2823-2830, 2000

S Gite, S Mamaev, J Olejnik, K Rothschild:
Ultrasensitive Fluorescence-Based Detection of Nascent Proteins in Gels.  Anal Biochem. 279 (2):  218-225, 2000

V Bergo, E Spudich, K Scott, JL Spudich, KJ Rothschild:
FTIR Analysis of the O-intermediate in sensory rhodopsin II:  Asp73 is the  Schiff base proton acceptor.  Biochemistry 39:  2823-2830, 2000

KJ Rothschild, S Gite:
tRNA-mediated protein engineering.  Curr. Opin. Biotechnol. 10:  64-70, 1999

F DeLange, CHW Klaassen, SE Wallace-Williams, PHM Bovee-Geurts, XM Liu, WJ DeGrip, KJ Rothschild:
Tyrosine structural changes during  photoactivation of rhodopsin.  J. Biol. Chem. 273:  23735-23739, 1998

P Rath, W DeGrip, K Rothschild:
Photoactivation Causes H/D Exchange in Core Region of Rhodopsin Structure.  Biophys. J. 74:  192-198, 1998

WB Fischer, P Wu, U Hoffman, KJ Eichhorn, HW Siesler, K Rothschild:
Comparison of Bacteriorhodpsin Conformational Changes with Uniaxially Elongated Aliphatic Polyamide Samples Using FT-IR Difference Spectroscopy.  Mikrochim. Acta 14:  437-439, 1997

JF Hunt, P Rath, KJ Rothschild, DM Engelman:
Spontaneous, pH-Dependent membrane Insertion of a Transbilayer alpha-Helix. 
Biochemistry 36:  15177-15192, 1997

P Rath, E Spudich, DD Neal, JL Spudich, KJ Rothschild:
Asp76 is the Schiff base counterion and proton acceptor in the proton- translocating form of sensory rhodopsin I.  Biochemistry 35:  6690-6696, 1996

CFC Ludlam, IT Arkin, XM Liu, MS Rothman, P Rath, S Aimoto, SO Smith, DM Engelman, KJ Rothschild:
Fourier Transform Infrared Spectroscopy and Site-Directed Isotope Labeling as a Probe of Local Secondary Structure in the Transmembrane Domain of Phospholamban.  Biophysical Journal 70:  1728-1736, 1996

J Olejnik, E Krzymanska-Olejnik, KJ Rothschild:
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.  Nucleic Acids Research 24:  361-366, 1996

P Rath, E Spudich, DD Neal, JL Spudich, KJ Rothschild:
Asp76 Is the Schiff Base Counterion and Proton Acceptor in the Proton-Translocating Form of Sensory Rhodopsin I.  Biochemistry 35:  6690-6696, 1996

IT Arkin, MS Rothman, CFC Ludlam, S Aimoto, DM Engelman, KJ Rothschild, SO Smith:
Structural Model of the Phospholamban Ion Channel Complex in Phospholipid Membranes.  J. Mol. Biol. 248:  824-834, 1995

XM Liu, S Sonar, CP Lee, M Coleman, UL RajBhandary, KJ Rothschild:
Site-directed isotope labeling and FTIR spectroscopy: Assignment of tyrosine bands in the bR=>M difference spectrum of bacteriorhodopsin.  Biophysical Chemistry 56:  63-70, 1995

CFC Ludlam, S Sonar, CP Lee, M Coleman, J Herzfeld, UL RajBhandary, KJ Rothschild: Site-directed isotope labeling and ATR-FTIR difference spectroscopy of bacteriorhodopsin: The peptide carbonyl group of Tyr 185 is structurally active during the bR=>N transition.  Biochemistry 34:  2-6, 1995

GLJ DeCaluwé, PHM Bovee-Geurts, P Rath, KJ Rothschild, WJ DeGrip:
Effect of carboxyl mutations on functional properties of bovine rhodopsin.  Biophysical Chemistry 56:  79-87, 1995

A Nilsson, P Rath, J Olejnik, M Coleman, KJ Rothschild:
Protein Conformational Changes during Bacteriorhodopsin Photocycle: A Fourier transform infrared/resonance Raman study of the alkaline form of the mutant Asp-85->Asn.  The Journal of Biological Chemistry 270:  29746-29751, 1995

S Sonar, CP Lee, M Coleman, N Patel, X Liu, T Marti, HG Khorana, UL Rajbhandary, K Rothschild:
Site-directed isotope labelling and FTIR spectroscopy of bacteriorhodopsin.  Nature Structural Biology 1:  512-517, 1995

C Safinya, KJ Rothschild:
Self-assembly and protein stability.  Nature 370:  105, 1994

Y Shen, CR Saffinya, KS Liang, AF Ruppert, KJ Rothschild:
Stabilization of the Membrane Protein Bacteriorhodopsin to 140 C in Two Dimensional Films.  Nature 366:  48-50, 1993

Book Contributions:

WJ DeGrip, KJ Rothschild:
Structure and Mechanism of Vertebrate Visual Pigments.  In Molecular Mechanisms in Visual Transduction. (Ed. Stravenga, D.G. et al.)  Elsevier, Amsterdam.  1-54, 2000

S Sonar, CP Lee, CFC Ludlam, XM Liu, M Coleman, T Marti, HG Khorana, UL RajBhandary, KJ Rothschild:
Site-Directed Isotope Labeling of Membrane Proteins:  A New Tool for Spectroscopists.  In Techniques in Protein Chemistry.  Academic Press.  151-159, 1996

KJ Rothschild, S Sonar:
Bacteriorhodopsin: New biophysical perspectives. In CRC Handbook of Organic Photochemistry and Photobiology.  CRC Press, Inc., London.  1521-1544, 1995