BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//RLASKEY//CALENDEROUS//EN
CALSCALE:GREGORIAN
METHOD:PUBLISH
BEGIN:VEVENT
DTSTAMP:20260815T064205Z
LAST-MODIFIED:20121116T203436Z
DTSTART:20100122T170000Z
DTEND:20100122T180000Z
UID:event538@bu.edu
URL:http://physics.bu.edu/internal/events/show/538
SUMMARY:Spectral reflectance biosensing for label-free and dynamic detectio
	n of protein\, DNA and viruses
DESCRIPTION:Featuring Bennett Goldberg\, Boston University\nHosted by: Clau
	dio Chamon\, Ophelia Tsui\n\nPart of the Biophysics/Condensed Matter Semina
	r Series.\n\nAbstract:\nT\nHE development of compact\, rapid\, multiplexed 
	and high-throughput molecular recognition platforms hold the promise of rev
	olutionizing personalized medicine\, point-of-care diagnostics and monitori
	ng\, and global health delivery in resource-limited settings.  The approach
	 of direct monitoring of primary molecular binding interactions obviates th
	e need for labeling or secondary reactants and thus has the greatest potent
	ial for broad applications. Spectral Reflectance Biosensing (SRB) is a simp
	le interferometric technique that monitors the optical phase difference res
	ulting from accumulated biomolecular mass on the surface of a chip. We have
	 demonstrated quantitative and dynamic measurements with ~3 pg/mm2 sensitiv
	ity for molecular interactions\, including protein-protein binding\, DNA-pr
	otein binding\, simultaneous detection of antigens and antibodies\, and DNA
	 hybridization kinetics. \nSRB is a common path optical interferometer and 
	so is completely insensitive to variations in concentration of analyte mate
	rial or temperature. Also\, SRB is independent of surface conformation of t
	he molecules because it does not rely on an evanescent wave interaction lik
	e Surface Plasmon Resonance.  Importantly\, this advantage allows utilizati
	on of unique polymeric coatings for immobilization and surface binding whic
	h results in versatile and high-density arrays. In addition to high-sensiti
	vity\, SRB has a very large field-of-view so the number of spots can be use
	d alternatively to optimize sensitivity and signal-to-noise or increase thr
	oughput. SRB can thus measure binding signals over hundreds of different co
	nditions simultaneously\, and be easily expanded to suit the needs of vario
	us screening protocols. Recently\, we have simplified the instrumentation b
	y utilizing discrete LED sources instead of a tunable laser\, improved spat
	ial resolutions and demonstrated capability in single pathogen (virus) dete
	ction.  \nReferences\n\n[1]I. E. Ozkumur J.W. Needham\, D. A. Bergstein\, R
	. Gonzalez\, M. Cabodi\, J. M. Gershoni\, B. B. Goldberg\, and M. S. Ünlü
	\, "Label-free and dynamic detection of biomolecular interactions for high-
	throughput microarray applications\," PNAS\, Vol. 105\, pp. 7988–7992 (20
	08)\n[2]I. E. Ozkumur\, A. Yalcin\, M. Cretich\, C. Lopez\, D. A. Bergstein
	\, B. B. Goldberg\, M. Chiari\, M. S.Ünlü\, "Quantification of DNA and pr
	otein adsorption by optical phase shift \," Biosensors and Bioelectronics\,
	 Vol. 25\, pp. 167-172\, (2009).\n[3]D. A. Bergstein\, I. E. Ozkumur\, A. C
	. Wu\, A. Yalcin\, J. R. Colson\, J. W. Needham\, R. J. Irani\, J. M. Gersh
	oni\, B. B. Goldberg\, C. DeLisi\, M. F. Ruane\, M. S. Ünlü\, "Resonant C
	avity Imaging: A Means Toward High-Throughput Label-Free Protein Detection\
	," IEEE J. Select. Topics in Quantum Electron.\, Special Issue on Biophoton
	ics\, vol. 14\, no. 1\, pp. 131-139\, (2008)\n[4]I. E. Ozkumur\, et al.\, "
	Label-free microarray imaging for direct detection of DNA hybridization and
	 single-nucleotide mismatches\," Biosensors and Bioelectronics\, accepted f
	or publication (2010)
LOCATION: \, \, 
STATUS:CONFIRMED
CLASS:PUBLIC
END:VEVENT
END:VCALENDAR