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DTSTAMP:20260815T210050Z
LAST-MODIFIED:20180116T154837Z
DTSTART:20180212T210000Z
DTEND:20180212T220000Z
UID:event1875@bu.edu
URL:http://physics.bu.edu/internal/events/show/1875
SUMMARY:"Single Molecules Come Into Focus: Understanding RNA-Driven Regulat
	ion From First Principles"
DESCRIPTION:Featuring Nils G. Walter\, University of Michigan\, Ann Arbor\n
	Hosted by: Maria Kamenetska\n\nPart of the Biophysics Seminars.\n\nNature a
	nd Nanotechnology likewise employ nanoscale machines that self-assemble int
	o structures of complex architecture and functionality.  Fluorescence micro
	scopy offers a non-invasive tool to probe and ultimately dissect and contro
	l these nanoassemblies in real-time.  In particular\, single molecule fluor
	escence resonance energy transfer (smFRET) allows us to measure distances a
	t the 2-8 nm scale\, whereas complementary super-resolution localization te
	chniques based on Gaussian fitting of imaged point spread functions (PSFs) 
	measure distances in the 10 nm and longer range.  First\, I will describe a
	 tool we developed termed Single Molecule Kinetic Analysis of RNA Transient
	 Structure (SiM-KARTS) that detects the conformational changes of single ri
	boswitch containing bacterial mRNAs upon sensing the metabolite preQ11.  Se
	cond\, I will illustrate how we demonstrated that ligand binding modulates 
	cross-coupling between riboswitch folding and transcriptional pausing by th
	e bacterial RNA polymerase.  Third\, I will describe a method for the intra
	cellular single molecule\, high-resolution localization and counting (iSHiR
	LoC) of microRNAs (miRNAs)\, a large group of gene silencers with profound 
	roles in our body\, from stem cell development to cancer2-6.  Single microi
	njected\, singly-fluorophore labeled\, functional miRNAs were tracked at su
	per-resolution within individual diffusing particles\, revealing their sub-
	cellular trafficking.  Finally\, I will show how Single-Molecule Recognitio
	n through Equilibrium Poisson Sampling (SiMREPS) can precisely count single
	 microRNA molecules in human blood serum as a new detection paradigm of cli
	nical relevance.
LOCATION:SCI 117\, 590 Commonwealth Avenue\, 02215
STATUS:CONFIRMED
CLASS:PUBLIC
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