Conjugate Adaptive Optics in Biomedical Microscopy
This event is part of the Departmental Seminars.
Dissertation Committee: Jerome Mertz, Shyam Erramilli, Pankaj Mehta, Thomas Bifano, Lei Tian (ECE)
The quality of biomedical microscopy imaging is often degraded by sample-induced aberrations. Adaptive optics (AO) is a standard approach to counter such aberrations. In common practice of AO, an active optical correction element, usually a deformable mirror (DM), is usually inserted in the pupil plane of the objective lens. However, the placement of the DM in a plane conjugate to a primary sample-induced aberration plane can be more advantageous, especially in situations where the aberrations are spatially varying and arise mainly from a dominant layer. In this talk I will describe two novel implementations of sensor-based conjugate AO, which do not require the use of guide stars. The first implementation is applied in wide-field microscopy of transmission mode. The wavefront sensing is based on a technique called partitioned aperture wavefront sensing that we have developed earlier. The second implementation is in fluorescent microscopy. The wavefront sensing strategy is based on oblique back-illumination. In Both implementations, I will address the key challenges of developing wavefront sensors that are capable of operating with uncollimated light. I will demonstrate that both implementations not only run efficiently in video-rate, but also provide large corrected field of view.